AUTHOR=Ling Shukuan , Zhong Guohui , Sun Weijia , Liang Fengji , Wu Feng , Li Hongxing , Li Yuheng , Zhao Dingsheng , Song Jinping , Jin Xiaoyan , Wu Xiaorui , Song Hailin , Li Qi , Li Yinghui , Chen Shanguang , Xiong Jianghui , Li Yingxian TITLE=Circulating microRNAs Correlated with Bone Loss Induced by 45 Days of Bed Rest JOURNAL=Frontiers in Physiology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2017.00069 DOI=10.3389/fphys.2017.00069 ISSN=1664-042X ABSTRACT=

The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with bone loss induced by bed rest, and testify whether the selected miRNAs could reflect the bone mineral status of human after bed-rest. We analyzed plasma miRNA levels of 16 subjects after 45 days of −6° head-down tilt bed rest, which is a reliable model for the simulation of microgravity. We characterize the circulating miRNA profile in individuals after bed rest and identify circulating miRNAs which can best reflect the level of bone loss induced by bed rest. Expression profiling of circulating miRNA revealed significant downregulation of 37 miRNAs and upregulation of 2 miRNAs, while only 11 of the downregulated miRNAs were further validated in a larger volunteer cohort using qPCR. We found that 10 of these 11 miRNAs (miR-103, 130a, 1234, 1290, 151-5p, 151-3p, 199a-3p, 20a, 363, and 451a) had ROC curve that distinguished the status after bed rest. Importantly, significant positive correlations were identified between bone loss parameters and several miRNAs, eventually miR-1234 showed clinical significance in detecting the bone loss of individuals after 45 days of bed rest.