AUTHOR=Abdelhamed Hossam , Lawrence Mark L. , Karsi Attila 

TITLE=The Role of TonB Gene in Edwardsiella ictaluri Virulence

JOURNAL=Frontiers in Physiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2017.01066

DOI=10.3389/fphys.2017.01066

ISSN=1664-042X

ABSTRACT=<p><italic>Edwardsiella ictaluri</italic> is a Gram-negative facultative intracellular pathogen that causes enteric septicemia in catfish (ESC). Stress factors including poor water quality, poor diet, rough handling, overcrowding, and water temperature fluctuations increase fish susceptibility to ESC. The TonB energy transducing system (TonB-ExbB-ExbD) and TonB-dependent transporters of Gram-negative bacteria support active transport of scarce resources including iron, an essential micronutrient for bacterial virulence. Deletion of the <italic>tonB</italic> gene attenuates virulence in several pathogenic bacteria. In the current study, the role of TonB (NT01EI_RS07425) in iron acquisition and <italic>E. ictaluri</italic> virulence were investigated. To accomplish this, the <italic>E. ictaluri tonB</italic> gene was in-frame deleted. Growth kinetics, iron utilization, and virulence of the <italic>Ei</italic>Δ<italic>tonB</italic> mutant were determined. Loss of TonB caused a significant reduction in bacterial growth in iron-depleted medium (<italic>p</italic> &gt; 0.05). The <italic>Ei</italic>Δ<italic>tonB</italic> mutant grew similarly to wild-type <italic>E. ictaluri</italic> when ferric iron was added to the iron-depleted medium. The <italic>Ei</italic>Δ<italic>tonB</italic> mutant was significantly attenuated in catfish compared with the parent strain (21.69 vs. 46.91% mortality). Catfish surviving infection with <italic>Ei</italic>Δ<italic>tonB</italic> had significant protection against ESC compared with naïve fish (100 vs. 40.47% survival). These findings indicate that TonB participates in pathogenesis of ESC and is an important <italic>E. ictaluri</italic> virulence factor.</p>