AUTHOR=Emami Shahram , Yee Muh-ching , Dinneny Jose R.

TITLE=A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology

JOURNAL=Frontiers in Plant Science

VOLUME=4

YEAR=2013

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2013.00339

DOI=10.3389/fpls.2013.00339

ISSN=1664-462X

ABSTRACT=<p>Tools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent innovations in molecular cloning methods has vastly expanded the repertoire with which plant biologists can engineer a transgene. Here we describe a new set of binary vectors for use in <italic>Agrobacterium</italic>-mediated plant transformation that utilizes the Golden-Gate Cloning approach. Our optimized protocol facilitates the rapid and inexpensive generation of multi-component transgenes for later introduction into plants.</p>