AUTHOR=Gujaria-Verma Neha , Vail Sally L. , Carrasquilla-Garcia Noelia , Penmetsa R. Varma , Cook Douglas R. , Farmer Andrew D. , Vandenberg Albert , Bett Kirstin E. 

TITLE=Genetic mapping of legume orthologs reveals high conservation of synteny between lentil species and the sequenced genomes of Medicago and chickpea

JOURNAL=Frontiers in Plant Science

VOLUME=5

YEAR=2014

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2014.00676

DOI=10.3389/fpls.2014.00676

ISSN=1664-462X

ABSTRACT=<p>Lentil <italic>(Lens culinaris</italic> Medik.) is a global food crop with increasing importance for food security in south Asia and other regions. <italic>Lens ervoides</italic>, a wild relative of cultivated lentil, is an important source of agronomic trait variation. <italic>Lens</italic> is a member of the galegoid clade of the Papilionoideae family, which includes other important dietary legumes such as chickpea (<italic>Cicer arietinum</italic>) and pea (<italic>Pisum sativum</italic>), and the sequenced model legume <italic>Medicago truncatula</italic>. Understanding the genetic structure of <italic>Lens</italic> spp. in relation to more fully sequenced legumes would allow leveraging of genomic resources. A set of 1107 TOG-based amplicons were identified in <italic>L. ervoides</italic> and a subset thereof used to design SNP markers for mapping. A map of <italic>L. ervoides</italic> consisting of 377 SNP markers spread across seven linkage groups was developed using a GoldenGate genotyping array and single SNP marker assays. Comparison with maps of <italic>M. truncatula</italic> and <italic>L. culinaris</italic> documented considerable shared synteny and led to the identification of a few major translocations and a major inversion that distinguish <italic>Lens</italic> from <italic>M. truncatula</italic>, as well as a translocation that distinguishes <italic>L. culinaris</italic> from <italic>L. ervoides</italic>. The identification of chromosome-level differences among Lens spp. will aid in the understanding of introgression of genes from <italic>L. ervoides</italic> into cultivated <italic>L. culinaris</italic>, furthering genetic research and breeding applications in lentil.</p>