AUTHOR=Zhao Minglei , Yang Songguang , Liu Xuncheng , Wu Keqiang 

TITLE=Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related genes

JOURNAL=Frontiers in Plant Science

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00159

DOI=10.3389/fpls.2015.00159

ISSN=1664-462X

ABSTRACT=<p>Seed dormancy controls germination and plays a critical role in regulating the beginning of the life cycle of plants. Seed dormancy is established and maintained during seed maturation and is gradually broken during dry storage (after-ripening). The plant hormone abscisic acid (ABA) and DELAY OF GERMINATION1 (DOG1) protein are essential regulators of seed dormancy. Recent studies revealed that chromatin modifications are also involved in the transcription regulation of seed dormancy. Here, we showed that two <italic>Arabidopsis</italic> histone demethylases, LYSINESPECIFIC DEMETHYLASE LIKE 1 and 2 (LDL1 and LDL2) act redundantly in repressing of seed dormancy. <italic>LDL1</italic> and <italic>LDL2</italic> are highly expressed in the early silique developing stage. The <italic>ldl1 ldl2</italic> double mutant displays increased seed dormancy, whereas overexpression of <italic>LDL1</italic> or <italic>LDL2</italic> in <italic>Arabidopsis</italic> causes reduced dormancy. Furthermore, we showed that <italic>LDL1</italic> and <italic>LDL2</italic> repress the expression of seed dormancy-related genes, including <italic>DOG1</italic>, <italic>ABA2</italic> and <italic>ABI3</italic> during seed dormancy establishment. Furthermore, genetic analysis revealed that the repression of seed dormancy by LDL1 and LDL2 requires <italic>DOG1</italic>, <italic>ABA2</italic>, and <italic>ABI3</italic>. Taken together, our findings revealed that LDL1 and LDL2 play an essential role in seed dormancy.</p>