AUTHOR=Bartels Benjamin , Svatoš Aleš 

TITLE=Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI

JOURNAL=Frontiers in Plant Science

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00471

DOI=10.3389/fpls.2015.00471

ISSN=1664-462X

ABSTRACT=<p>This short review aims to summarize the current developments and applications of mass spectrometry-based methods for <italic>in situ</italic> profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization and UV-laser desorption/ionization methods are reviewed. The underlying mechanisms of the ionization techniques–namely, laser ablation of biological samples and electrospray ionization–as well as variations of the LAESI ion source for specific targets of interest are described.</p>