AUTHOR=Dogra Vivek , Bagler Ganesh , Sreenivasulu Yelam 

TITLE=Re-analysis of protein data reveals the germination pathway and up accumulation mechanism of cell wall hydrolases during the radicle protrusion step of seed germination in Podophyllum hexandrum- a high altitude plant

JOURNAL=Frontiers in Plant Science

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00874

DOI=10.3389/fpls.2015.00874

ISSN=1664-462X

ABSTRACT=<p><italic>Podophyllum hexandrum</italic> Royle is an important high-altitude plant of Himalayas with immense medicinal value. Earlier, it was reported that the cell wall hydrolases were up accumulated during radicle protrusion step of <italic>Podophyllum</italic> seed germination. In the present study, <italic>Podophyllum</italic> seed Germination protein interaction Network (PGN) was constructed by using the differentially accumulated protein (DAP) data set of <italic>Podophyllum</italic> during the radicle protrusion step of seed germination, with reference to <italic>Arabidopsis</italic> protein–protein interaction network (<italic>At</italic>PIN). The developed PGN is comprised of a giant cluster with 1028 proteins having 10,519 interactions and a few small clusters with relevant gene ontological signatures. In this analysis, a germination pathway related cluster which is also central to the topology and information dynamics of PGN was obtained with a set of 60 key proteins. Among these, eight proteins which are known to be involved in signaling, metabolism, protein modification, cell wall modification, and cell cycle regulation processes were found commonly highlighted in both the proteomic and interactome analysis. The systems-level analysis of PGN identified the key proteins involved in radicle protrusion step of seed germination in <italic>Podophyllum</italic>.</p>