AUTHOR=Li Dandan , Xu Xuemei , Hu Xiaoqing , Liu Quangang , Wang Zhanchao , Zhang Haizhen , Wang Han , Wei Ming , Wang Hanzeng , Liu Haimei , Li Chenghao 

TITLE=Genome-Wide Analysis and Heavy Metal-Induced Expression Profiling of the HMA Gene Family in Populus trichocarpa

JOURNAL=Frontiers in Plant Science

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.01149

DOI=10.3389/fpls.2015.01149

ISSN=1664-462X

ABSTRACT=<p>The heavy metal ATPase (HMA) family plays an important role in transition metal transport in plants. However, this gene family has not been extensively studied in <italic>Populus trichocarpa</italic>. We identified 17 HMA genes in <italic>P. trichocarpa</italic> (<italic>PtHMA</italic>s), of which <italic>PtHMA1–PtHMA4</italic> belonged to the zinc (Zn)/cobalt (Co)/cadmium (Cd)/lead (Pb) subgroup, and <italic>PtHMA5–PtHMA8</italic> were members of the copper (Cu)/silver (Ag) subgroup. Most of the genes were localized to chromosomes I and III. Gene structure, gene chromosomal location, and synteny analyses of <italic>PtHMA</italic>s indicated that tandem and segmental duplications likely contributed to the expansion and evolution of the <italic>PtHMA</italic>s. Most of the HMA genes contained abiotic stress-related <italic>cis</italic>-elements. Tissue-specific expression of <italic>PtHMA</italic> genes showed that <italic>PtHMA1</italic> and <italic>PtHMA4</italic> had relatively high expression levels in the leaves, whereas Cu/Ag subgroup (<italic>PtHMA5.1- PtHMA8</italic>) genes were upregulated in the roots. High concentrations of Cu, Ag, Zn, Cd, Co, Pb, and Mn differentially regulated the expression of <italic>PtHMAs</italic> in various tissues. The preliminary results of the present study generated basic information on the HMA family of <italic>Populus</italic> that may serve as foundation for future functional studies.</p>