AUTHOR=Corbin Jasmine M. , Hashimoto Bryce I. , Karuppanan Kalimuthu , Kyser Zachary R. , Wu Liying , Roberts Brian A. , Noe Amy R. , Rodriguez Raymond L. , McDonald Karen A. , Nandi Somen TITLE=Semicontinuous Bioreactor Production of Recombinant Butyrylcholinesterase in Transgenic Rice Cell Suspension Cultures JOURNAL=Frontiers in Plant Science VOLUME=7 YEAR=2016 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00412 DOI=10.3389/fpls.2016.00412 ISSN=1664-462X ABSTRACT=

An active and tetrameric form of recombinant butyrylcholinesterase (BChE), a large and complex human enzyme, was produced via semicontinuous operation in a transgenic rice cell suspension culture. After transformation of rice callus and screening of transformants, the cultures were scaled up from culture flask to a lab scale bioreactor. The bioreactor was operated through two phases each of growth and expression. The cells were able to produce BChE during both expression phases, with a maximum yield of 1.6 mg BChE/L of culture during the second expression phase. Cells successfully regrew during a 5-day growth phase. A combination of activity assays and Western blot analysis indicated production of an active and fully assembled tetramer of BChE.