AUTHOR=Deng Zhi , Chen Jiangshu , Leclercq Julie , Zhou Zhuangzhi , Liu Changren , Liu Hui , Yang Hong , Montoro Pascal , Xia Zhihui , Li Dejun 

TITLE=Expression Profiles, Characterization and Function of HbTCTP in Rubber Tree (Hevea brasiliensis)

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00789

DOI=10.3389/fpls.2016.00789

ISSN=1664-462X

ABSTRACT=<p>As a highly conserved protein, the translationally controlled tumor protein (TCTP) carries out vital roles in various life processes. In rubber tree, two TCTP genes, <italic>HbTCTP</italic> and <italic>HbTCTP1</italic>, were cloned, but only <italic>HbTCTP1</italic> was studied in details. In this study, <italic>cis</italic>-acting regulatory elements, expression patterns, subcellular localization, interacting proteins, and antioxidant activity of <italic>HbTCTP</italic> were systematically analyzed. Besides the common <italic>cis</italic>-acting regulatory elements, <italic>HbTCTP</italic> promoter also harbored various known <italic>cis</italic>-elements that respond to hormone/stresses. Being consistent with the aforementioned results, <italic>HbTCTP</italic> was regulated by drought, low temperature, high salt, ethylene (ET), wounding, H<sub>2</sub>O<sub>2</sub>, and methyl jasmonate (MeJA) treatments. <italic>HbTCTP</italic> was expressed throughout different tissues and developmental stages of leaves. In addition, <italic>HbTCTP</italic> was associated with tapping panel dryness (TPD). HbTCTP was localized in the membrane, cytoplasm and the nucleus, and interacted with four proteins rubber elongation factor (REF), 17.5 kDa heat shock family protein, annexin, and REF-like stress related protein 1. Being similar to HbTCTP1, HbTCTP also indicated antioxidant activity in metal-catalyzed oxidation (MCO) system. Our results are useful for further understanding the molecular characterization and expression profiles of <italic>HbTCTP</italic>, but also lay a solid foundation for elucidating the function of <italic>HbTCTP</italic> in rubber tree.</p>