AUTHOR=Liu Zhongyuan , Alseekh Saleh , Brotman Yariv , Zheng Yi , Fei Zhangjun , Tieman Denise M. , Giovannoni James J. , Fernie Alisdair R. , Klee Harry J. 

TITLE=Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.01671

DOI=10.3389/fpls.2016.01671

ISSN=1664-462X

ABSTRACT=<p>A major resource for tomato quality improvement and gene discovery is the collection of introgression lines (ILs) of cultivated <italic>Solanum lycopersicum</italic> that contain different, defined chromosomal segments derived from the wild tomato relative, <italic>S. pennellii.</italic> Among these lines, IL4-4, in which the bottom of <italic>S. lycopersicum</italic> (cv. M82) chromosome 4 is replaced by the corresponding <italic>S. pennellii</italic> segment, is altered in many primary and secondary metabolites, including many related to fruit flavor and nutritional quality. Here, we provide a comprehensive profile of IL4-4 ripe fruit metabolites, the transcriptome and fine mapping of sub-ILs. Remarkably, out of 327 quantified metabolites, 185 were significantly changed in IL4-4 fruit, compared to the control. These altered metabolites include volatile organic compounds, primary and secondary metabolites. Partial least squares enhanced discriminant analysis of the metabolite levels among sub-ILs indicated that a genome region encompassing 20 putative open reading frames is responsible for most of the metabolic changes in IL4-4 fruit. This work provides comprehensive insights into IL4-4 fruit biochemistry, identifying a small region of the genome that has major effects on a large and diverse set of metabolites.</p>