AUTHOR=Yang Chunxiao , Preisser Evan L. , Zhang Hongjun , Liu Yong , Dai Liangying , Pan Huipeng , Zhou Xuguo TITLE=Selection of Reference Genes for RT-qPCR Analysis in Coccinella septempunctata to Assess Un-intended Effects of RNAi Transgenic Plants JOURNAL=Frontiers in Plant Science VOLUME=7 YEAR=2016 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.01672 DOI=10.3389/fpls.2016.01672 ISSN=1664-462X ABSTRACT=
The development of genetically engineered plants that employ RNA interference (RNAi) to suppress invertebrate pests opens up new avenues for insect control. While this biotechnology shows tremendous promise, the potential for both non-target and off-target impacts, which likely manifest via altered mRNA expression in the exposed organisms, remains a major concern. One powerful tool for the analysis of these un-intended effects is reverse transcriptase-quantitative polymerase chain reaction, a technique for quantifying gene expression using a suite of reference genes for normalization. The seven-spotted ladybeetle