AUTHOR=Larkan Nicholas J. , Yu Fengqun , Lydiate Derek J. , Rimmer S. Roger , Borhan M. Hossein 

TITLE=Single R Gene Introgression Lines for Accurate Dissection of the Brassica - Leptosphaeria Pathosystem

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.01771

DOI=10.3389/fpls.2016.01771

ISSN=1664-462X

ABSTRACT=<p>Seven blackleg resistance (<italic>R</italic>) genes (<italic>Rlm1, Rlm2, Rlm3, Rlm4, LepR1, LepR2</italic> &amp; <italic>LepR3</italic>) were each introgressed into a common susceptible <italic>B. napus</italic> doubled-haploid (DH) line through reciprocal back-crossing, producing single-<italic>R</italic> gene introgression lines (ILs) for use in the pathological and molecular study of <italic>Brassica</italic>—<italic>Leptosphaeria</italic> interactions. The genomic positions of the <italic>R</italic> genes were defined through molecular mapping and analysis with transgenic <italic>L. maculans</italic> isolates was used to confirm the identity of the introgressed genes where possible. Using <italic>L. maculans</italic> isolates of contrasting avirulence gene (<italic>Avr</italic>) profiles, we preformed extensive differential pathology for phenotypic comparison of the ILs to other <italic>B. napus</italic> varieties, demonstrating the ILs can provide for the accurate assessment of <italic>Avr</italic>-<italic>R</italic> gene interactions by avoiding non-<italic>Avr</italic> dependant alterations to resistance responses which can occur in some commonly used <italic>B. napus</italic> varieties. Whole-genome SNP-based assessment allowed us to define the donor parent introgressions in each IL and provide a strong basis for comparative molecular dissection of the pathosystem.</p>