AUTHOR=Liu Xing , Han Fengqing , Kong Congcong , Fang Zhiyuan , Yang Limei , Zhang Yangyong , Zhuang Mu , Liu Yumei , Li Zhansheng , Lv Honghao TITLE=Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection JOURNAL=Frontiers in Plant Science VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00354 DOI=10.3389/fpls.2017.00354 ISSN=1664-462X ABSTRACT=
Cabbage is an economically important vegetable worldwide. Cabbage Fusarium Wilt (CFW) is a destructive disease that results in considerable yield and quality losses in cole crops. The use of CFW-resistant varieties is the most effective strategy to mitigate the effects of CFW. 01-20 is an elite cabbage line with desirable traits and a high combining ability, but it is highly susceptible to CFW. To rapidly transfer a CFW resistance gene into 01-20 plants, we used microspore cultures to develop 230 doubled haploid (DH) lines from a cross between 01-20 (highly susceptible) and 96-100 (highly resistant). One of the generated DH lines (i.e., D134) was highly resistant to CFW and exhibited a phenotypic performance that was similar to that of line 01-20. Therefore, D134 was applied as the resistance donor parent. We generated 24 insertion–deletion markers using whole genome resequencing data for lines 01-20 and 96-100 to analyze the genomic backgrounds of backcross (BC) progenies. Based on the CFW resistance gene