AUTHOR=Niu Jingping , Guo Na , Sun Jutao , Li Lihong , Cao Yongce , Li Shuguang , Huang Jianli , Zhao Jinming , Zhao Tuanjie , Xing Han 

TITLE=Fine Mapping of a Resistance Gene RpsHN that Controls Phytophthora sojae Using Recombinant Inbred Lines and Secondary Populations

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00538

DOI=10.3389/fpls.2017.00538

ISSN=1664-462X

ABSTRACT=<p>Phytophthora root rot (PRR), caused by <italic>Phytophthora sojae</italic>, has negative effects on soybean yield in China and can be controlled by identifying germplasm resources with resistance genes. In this study, the resistance locus <italic>RpsHN</italic> in the soybean line Meng8206 was mapped using two mapping populations. Initial mapping was realized using two recombinant inbred line (RIL) populations and included 103 F<sub>6:8</sub> RILs derived from a cross of Meng8206 × Linhedafenqing, including 2600 bin markers, and 130 F<sub>6:8</sub> RILs derived from a cross of Meng8206 × Zhengyang148, including 2267 bin markers. Subsequently, a 159 F<sub>2:3</sub> secondary population derived from a cross of Meng8206 × Linmeng6-46, were used to fine map this locus using SSR markers. Finally, the resistance locus from Meng8206 was fine mapped to a 278.7 kb genomic region flanked by SSR markers SSRSOYN-25 and SSRSOYN-44 at a genetic distance of 1.6 and 1.0 cM on chromosome 3 (Chr. 03). Real-time RT-PCR analysis of the possible candidate genes showed that three genes (<italic>Glyma.03g04260, Glyma.03g04300</italic>, and <italic>Glyma.03g04340</italic>) are likely involved in PRR resistance. These results will serve as a basis for cloning, transferring of resistant genes and breeding of <italic>P. sojae</italic>-resistant soybean cultivars through marker-assisted selection.</p>