AUTHOR=Li Mingwan , Chen Sufang , Zhou Renchao , Fan Qiang , Li Feifei , Liao Wenbo 

TITLE=Molecular Evidence for Natural Hybridization between Cotoneaster dielsianus and C. glaucophyllus

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00704

DOI=10.3389/fpls.2017.00704

ISSN=1664-462X

ABSTRACT=<p>Hybridization accompanied by polyploidization and apomixis has been demonstrated as a driving force in the evolution and speciation of many plants. A good example to study the evolutionary process of hybridization associated with polyploidy and apomixis is the genus <italic>Cotoneaster</italic> (Rosaceae), which includes approximately 150 species, most of which are polyploid apomicts. In this study, we investigated all <italic>Cotoneaster</italic> taxa distributed in a small region of Malipo, Yunnan, China. Based on the morphological characteristics, four <italic>Cotoneaster</italic> taxa were identified and sampled: <italic>C. dielsianus, C. glaucophyllus, C. franchetii</italic>, and a putative hybrid. Flow cytometry analyses showed that <italic>C. glaucophyllus</italic> was diploid, while the other three taxa were tetraploid. A total of five low-copy nuclear genes and six chloroplast regions were sequenced to validate the status of the putative hybrid. Sequence analyses showed that <italic>C. dielsianus</italic> and <italic>C. glaucophyllus</italic> are distantly related and they could be well separated using totally 50 fixed nucleotide substitutions and four fixed indels at the 11 investigated genes. All individuals of the putative hybrid harbored identical sequences: they showed chromatogram additivity for all fixed differences between <italic>C. dielsianus</italic> and <italic>C. glaucophyllus</italic> at the five nuclear genes, and were identical with <italic>C. glaucophyllus</italic> at the six chloroplast regions. Haplotype analysis revealed that <italic>C. dielsianus</italic> possessed nine haplotypes for the 11 genes, while <italic>C. glaucophyllus</italic> had ten, and there were no shared haplotypes between the two species. The putative hybrid harbored two haplotypes for each nuclear gene: one shared with <italic>C. dielsianus</italic> and the other with <italic>C. glaucophyllus</italic>. They possessed the same chloroplast haplotype with <italic>C. glaucophyllus</italic>. Our study provided convincing evidence for natural hybridization between <italic>C. dielsianus</italic> and <italic>C. glaucophyllus</italic>, and revealed that all hybrid individuals were derivatives of one initial F1 via apomixes. <italic>C. glaucophyllus</italic> served as the maternal parent at the initial hybridization event. We proposed that anthropological disturbance provided an opportunity for hybridization between <italic>C. dielsianus</italic> and <italic>C. glaucophyllus</italic>, and a tetraploid F1 successfully bred many identical progenies via apomixis. Under this situation, species integrity could be maintained for these <italic>Cotoneaster</italic> species, but attentions should be kept for this new-born hybrid.</p>