AUTHOR=Chen Piaojuan , Li Yi , Zhao Lihua , Hou Zhimin , Yan Maokai , Hu Bingyan , Liu Yanhui , Azam Syed Muhammad , Zhang Ziyan , Rahman Zia ur , Liu Liping , Qin Yuan 

TITLE=Genome-Wide Identification and Expression Profiling of ATP-Binding Cassette (ABC) Transporter Gene Family in Pineapple (Ananas comosus (L.) Merr.) Reveal the Role of AcABCG38 in Pollen Development

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.02150

DOI=10.3389/fpls.2017.02150

ISSN=1664-462X

ABSTRACT=<p>Pineapple (<italic>Ananas comosus L</italic>.) cultivation commonly relies on asexual reproduction which is easily impeded by many factors in agriculture production. Sexual reproduction might be a novel approach to improve the pineapple planting. However, genes controlling pineapple sexual reproduction are still remain elusive. In different organisms a conserved superfamily proteins known as ATP binding cassette (ABC) participate in various biological processes. Whereas, till today the <italic>ABC</italic> gene family has not been identified in pineapple. Here 100 <italic>ABC</italic> genes were identified in the pineapple genome and grouped into eight subfamilies (5 <italic>ABCAs</italic>, 20 <italic>ABCB</italic>s, 16 <italic>ABCCs</italic>, 2 <italic>ABCDs</italic>, one <italic>ABCEs</italic>, 5 <italic>ABCFs</italic>, 42 <italic>ABCGs</italic> and 9 <italic>ABCIs</italic>). Gene expression profiling revealed the dynamic expression pattern of <italic>ABC</italic> gene family in various tissues and different developmental stages. <italic>AcABCA5, AcABCB6, AcABCC4</italic>, <italic>AcABCC7</italic>, <italic>AcABCC9</italic>, <italic>AcABCG26</italic>, <italic>AcABCG38</italic> and <italic>AcABCG42</italic> exhibited preferential expression in ovule and stamen. Over-expression of <italic>AcABCG38</italic> in the <italic>Arabidopsis</italic> double mutant <italic>abcg1-2abcg16-2</italic> partially restored its pollen abortion defects, indicating that <italic>AcABCG38</italic> plays important roles in pollen development. Our study on <italic>ABC</italic> gene family in pineapple provides useful information for developing sexual pineapple plantation which could be utilized to improve pineapple agricultural production.</p>